Neb Molarity Calculator (2024)

1. Biomath Calculators | DNA Calculator | Vector Insert Ratio

DNA calculations to convert µg to pmol for double-stranded and single-stranded DNA, convert micrograms of DNA to pmol ends, calculate vector:insert molar ...
DNA calculations to convert µg to pmol for double-stranded and single-stranded DNA, convert micrograms of DNA to pmol ends, calculate vector:insert molar ratio and convert OD260 readings to µg/ml. Also calculate molarity of solutions, perform molar conversions, calculate dilutions and perform other calculations common in molecular biology labs.

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2. NEB Interactive Tools - New England Biolabs GmbH

Use this tool for your scientific calculations and conversions for DNA and RNA. Options include conversion of mass to moles, ligation amounts, conversion of OD ...
Home > Technical Resources NEB > NEB Interactive Tools

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3. In-Fusion molar ratio calculator - Takara Bio

In-Fusion molar ratio calculator. Calculating the optimal amounts of vector and insert to use in the In-Fusion Cloning reaction is easy with our In-Fusion molar ...
Calculate the optimal amounts of vector and insert for an In-Fusion Cloning reaction. Just enter the sizes of both vector and insert(s), in base pairs.

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4. NEBioCalculator | Best of The Web

15 apr 2014 · http://nebiocalculator.neb.com. Rating ... calculators (concentration from optical density, dilution calculator, and molarity calculator).
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In today’s world of tablets and smartphones, the NEBioCalculator may at first seem like it belongs under the “app review” section of this column. However, let’s not forget that bench scientists actually use their computers once in a while, too… The NEBioCalculator, an online tool from (you guessed it) New England Biolabs, provides a simple web interface for scientists to access a number of calculators relevant to molecular biology experiments. These include four DNA calculators (ligation calculator and three unit conversion calculators for double-stranded DNA), two RNA calculators (mass-to-moles and moles-to-mass conversions), and three general calculators (concentration from optical density, dilution calculator, and molarity calculator). The site is sleekly and minimally designed, thereby facilitating quick use of the calculators. There may not be a great depth of content here, but the tools that are on the site are quite useful.

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5. Ligation Calculator

7 dagen geleden · Our ligation calculator is an easy way to assess the optimal mass of both the insert and the vector needed in a cloning reaction.
Our ligation calculator is an easy way to assess the optimal mass of both the insert and the vector needed in a cloning reaction.

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6. Ligation Calculator - QIAGEN

This tool determines the mass of insert needed for various inserts:vector molar ratios within the range suitable for typical ligation reactions. Insert DNA ...
T4 DNA Ligase (Rapid)

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7. DNA Copy Number Calculator | Thermo Fisher Scientific - US

DNA Copy Number and Dilution Calculator · If you know that the weight of your DNA (molar mass per bp) is different from the average weight of DNA basepair, ...
This calculator provides instructions on how to dilute a DNA stock solution to obtain specific DNA copy number per μL.
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8. Pooling Calculator - Support Illumina

Pooling Calculator. Library Plexity. Do the libraries have the same concentration? Same. Different. Different, import values. Different, import values and names.
Library Plexity

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9. Molarity Calculator - GraphPad

Molarity Calculator · 1. Mass from volume & concentration. Concentration: Formula Weight (daltons):. Volume: Mass = · 2. Volume from mass & concentration. Mass:.

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10. NEBioCalculator: New online tool offers support for molecular biology ...

29 jan 2014 · ... molarity calculation, OD260 conversion, ligation calculation ... NEBuilder®, NEBaseChanger™, and its Tm Calculator. Unlike other web ...
NEBioCalculator, a new online "conversions and calculations" tool developed by New England Biolabs, offers bench-side support for molecular biology experimental planning.

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11. New England Biolabs launches NEBioCalculator - PR Newswire

28 jan 2014 · ... molarity calculation, OD260 conversion, ligation calculation ... NEBuilder®, NEBaseChanger™, and its Tm Calculator. Continue Reading.
/PRNewswire/ -- NEBioCalculator, a new online "conversions and calculations" tool developed by New England Biolabs (NEB®), offers bench-side support for...

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12. [PDF] Molecular Cloning - New England Biolabs GmbH

* Tm values should be determined using the NEB Tm calculator (TmCalculator.neb.com). ... • Vary the molar ratio of vector to insert from 1:1 to 1:10.

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13. Oligo Resuspension Calculator | LGC Biosearch Technologies

Easily create a stock solution by allowing the resuspension calculator take the guesswork out of dissolving your oligo ... Desired stock concentration. micromolar ...
Easily create a stock solution by allowing the resuspension calculator take the guesswork out of dissolving your oligo.

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14. Converting ng/µl to nM when calculating dsDNA library ...

6 mei 2024 · Determine the average size of the library by running it on an Agilent Technologies 2100 Bioanalyzer. Use the following formula to convert from ...
Some standard Illumina libraries, such as Nextera, require the use of dsDNA-specific fluorescent dye methods for accurate quantification. These methods typically measure dsDNA concentration in ng/µl. To convert from ng/µl to nM for cluster generation, follow the instructions below.

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15. [PDF] RAD-seq Protocol - bioRxiv

27 mrt 2019 · 0.5µl 10X NEB Buffer (the same as used for digestion). 1.5µl ... I used the molarity calculator provided in the following publication:.

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FAQs

What is the easiest way to calculate molarity? ›

So molarity is defined as moles of solute in moles divided by volume of solution in liters. Or as an equation M = n/V, where M is molarity, n is moles of solute, and V is volume. If you multiply the equation by volume you have a way to solve for number of moles, or MV = n.

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How to calculate molarity of DNA? ›

To determine the molarity of DNA molecules, dived the deoxyribonucleotides' molarity by [size of the DNA molecule (in bp) * 2]. The method for determination of DNA molecules' molarity is applicable for conditions where all of the DNA molecules in the sample have a single size.

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How do you calculate the molar mass of DNA? ›

The molecular weight or molar mass of any double-stranded DNA fragment can therefore be calculated by multiplying its length (in bp) by 650 and the answer will be expressed as daltons or g/mol.

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How to calculate moles of protein? ›

Divide the concentration (mg/ml) by the molecular weight. Astelia Francois, since you have 4 mL of 0.6 mG/mL (the total mass of protein = 4 x 0.6 mG = 2.4 mG of protein). Since the molecular weight is 18,000 Da, and moles equals mass/Molecular Weight), this equals (2.4/18,000) i.e., 0.000133 Moles of protein.

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What is the formula for molarity trick? ›

Calculating Molarity with Moles and Volume

Molarity is equal to the number of moles of a solute divided by the volume of the solution in liters. As such, it is written as: molarity = moles of solute / liters of solution.

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What is the shortcut to find molarity? ›

% by weight x 10xd Molarity = GMM where d is density and GMM is gram molecular mass. Derive the formula : Molarity = (% by weight × 10 × d )/ GMM Here d is density and GMM is gram molecular mass . The molarity of HNO3 in a sample which has density 1.4 g/mL and mass percentage of 63% is (Molecular weight of HNO3=63).

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Which of the formula is used to calculate molar mass? ›

Molar mass = mass/mole = g/mol

This leads to two important facts. The mass of one atom of carbon-12 the atomic mass of carbon-12 is exactly 12 atomic mass units. The mass of one mole of carbon-12 atoms is exactly 12 grams; its molar mass is exactly 12 grams per mole.

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What is the molar concentration of DNA in a human cell formula? ›

Hence, the molar concentration of DNA in each diploid cell in humans is 2.77 × 10 ²³ moles.

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What is the formula for mass concentration to molar concentration? ›

Decide on the mass concentration of your substance. Let's assume you have 5 g of HCl in a 1.2 liter solution. Convert the expressions above to obtain a molarity formula. As mass/volume = molarity × molar mass , then mass / (volume × molar mass) = molarity .

How to convert mg/mL into micromolar? ›

Divide by the molecular weight and multiply by 1000 mL. Leave the weight as mg and molecular weight in g/mol which will give mM directly.

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What is the correct formula to calculate moles? ›

To calculate the number of moles of any substance in the sample, we simply divide the given weight of the substance by its molar mass.

What is molarity how it is calculated? ›

Molarity is a unit of concentration expressed as the number of moles of dissolved solute per liter of solution. If the number of moles and the volume are divided by 1000, then molarity is expressed as the number of millimoles per milliliter of solution.

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How do you calculate the molarity of a solution concentration? ›

The molar concentration, also known as molarity, is the amount of moles of a solute per liter of solution. It can be calculated using the following formula: C = molar concentration in mol/L or M.

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How do you calculate molality? ›

The formula for molality is m = moles of solute / kilograms of solvent. In problem solving involving molality, we sometimes need to use additional formulas to get to the final answer. One formula we need to be aware of is the formula for density, which is d = m / v, where d is density, m is mass and v is volume.

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